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N3254

Sigma-Aldrich

4-Nitrophenyl phosphate di(tris) salt

phosphatase substrate

Synonym(s):

4-Nitrophenyl phosphate bis(tris) salt

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About This Item

Linear Formula:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS Number:
68189-42-4
Molecular Weight:
461.36
Beilstein/REAXYS Number:
1578142
EC Number:
269-198-6
MDL number:
MFCD00037009
UNSPSC Code:
12352204
PubChem Substance ID:
24897633
NACRES:
NA.83

Quality Level

100

assay

≥97% (enzymatic)

form

powder

solubility

water: 50 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

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Application

4-Nitrophenyl phosphate di(tris) salt has been used:
  • as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
  • to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
  • for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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