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N0142

Sigma-Aldrich

Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody produced in mouse

clone N52, ascites fluid

Synonym(s):

Anti-CMT2CC, Anti-NFH

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About This Item

MDL number:
MFCD00164708
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

N52, monoclonal

contains

15 mM sodium azide

species reactivity

wide range

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): 1:400 using rat cerebellum
microarray: suitable
western blot: 1:1,000 using rat brain extract

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)

General description

Neurofilaments are composed of three subunits, namely NEFL (light), NEFM (medium) and NEFH (heavy). NEFH (neurofilament heavy) gene encodes the most important neuron-specific intermediate filament of cytoskeleton in myelinated axons. This gene is mapped to human chromosome 22q12.2.
Monoclonal Anti-Neurofilament 200 (phosphorylated and non-phosphorylated) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

Displays a broad species cross-reactivity, recognizing both the phosphorylated and non-phosphorylated forms of this neural specific antigen using various techniques.

Immunogen

C-terminal segment of enzymatically dephosphorylated pig neurofilament 200.

Application

Monoclonal Anti-Neurofilament 200 (Phos. and Non-Phos.) antibody has been used in immunohistochemistry and western blotting.

Biochem/physiol Actions

The heavy polypeptide neurofilament encoded by neurofilament heavy chain (NEFH) gene participates in the maintenance of cytoskeleton and axonal architecture in the proximal axonal region of spinal motoneurons. Mutations in the NEFH gene has a key role in the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS). NEFH gene is also identified to generate autosomal dominant axonal Charcot-Marie-tooth disease (CMT2cc).

Physical form

Supplied as ascites fluid containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lydia Jiménez-Díaz et al.
PloS one, 3(4), e1961-e1961 (2008-04-10)
Recent studies have demonstrated the importance of local protein synthesis for neuronal plasticity. In particular, local mRNA translation through the mammalian target of rapamycin (mTOR) has been shown to play a key role in regulating dendrite excitability and modulating long-term
Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-ε-caprolactone and a collagen/poly-ε-caprolactone blend.
Schnell E, et al.
Biomaterials, 28(19), 3012-3025 (2007)
Clinical and genetic basis of familial amyotrophic lateral sclerosis.
Souza PVSD, et al.
Arquivos de Neuro-Psiquiatria, 73(12), 1026-1037 (2015)
Cryptic amyloidogenic elements in mutant NEFH causing Charcot-Marie-Tooth 2 trigger aggresome formation and neuronal death.
Jacquier A< et al.
Acta Neuropathologica Communications, 5(1), 55-55 (2017)
Fibrin/Schwann cell matrix in poly-epsilon-caprolactone conduits enhances guided nerve regeneration.
Galla TJ, et al.
The International Journal of Artificial Organs, 27(2), 127-136 (2004)
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