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L7275

Sigma-Aldrich

LB Broth (Lennox)

Tablet microbial growth medium

Synonym(s):

Lennox broth , Luria Bertani Broth

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

grade

for molecular biology

Quality Level

200

sterility

non-sterile

form

tablet

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

Dehydrated Luria-Bertani (LB) broth is called the Lennox LB, which has amino acids as carbon source.
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Application

LB Broth (Lennox) has been used for culturing Escherichia coli and bacterial artificial chromosomes (BACs).
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Lennox LB tablets provide:
  • Convenient small package to eliminate weighing
  • Fast preparation – no stirring required

Components

10 g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents

Reconstitution

Add each 1.1g tablet in 48.3mL water (no stirring required). Autoclave for 20 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Legal Information

Zeocin is a registered trademark of Cayla Sarl

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Escherichia coli Physiology in Luria-Bertani Broth
Sezonov G, et al.
Journal of Bacteriology, 189(23), 8746-8746 (2007)
Synthesis of antibacterial surfaces by plasma grafting of zinc oxide based nanocomposites onto polypropylene
De Rancourt Y, et al.
Journal of Colloid and Interface Science, 402, 320-326 (2013)
In Vitro packaging of λ dam vectors and their use in cloning DNA fragments.
Enquist, L. and Sternberg, N.
Methods in Enzymology, 68, 281-281 (1971)
Hybridization between Escherichia coli and Shigella.
Luria, S.E. and Burrous, J.W.
Journal of Bacteriology, 74, 281-281 (1957)
Fluorescence in situ hybridization of ductal lavage samples identifies malignant phenotypes from cytologically normal cells in women with breast cancer
Adduci KM, et al.
Cancer Cytopathology, 111(3), 185-191 (2007)
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Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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