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L2643

Sigma-Aldrich

α-Lactose monohydrate

suitable for cell culture, BioReagent

Synonym(s):

β-D-Gal-(1→4)-α-D-Glc, 4-O-β-D-Galactopyranosyl-α-D-glucose, Milk sugar

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About This Item

Empirical Formula (Hill Notation):
C12H22O11 · H2O
CAS Number:
5989-81-1
Molecular Weight:
360.31
MDL number:
MFCD00150747
UNSPSC Code:
12352201
PubChem Substance ID:
24896320
NACRES:
NA.75

biological source

bovine milk

Quality Level

200

product line

BioReagent

assay

≥99% total lactose basis (GC)

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

≤0.05% Glucose
≤4% β-lactose

solubility

water: 100 mg/mL, clear, colorless

SMILES string

[H]O[H].OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C12H22O11.H2O/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17;/h3-20H,1-2H2;1H2/t3-,4-,5+,6+,7-,8-,9-,10-,11+,12+;/m1./s1

InChI key

WSVLPVUVIUVCRA-KPKNDVKVSA-N

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Application

α-Lactose monohydrate has been used:
  • as a component of fraction B of diluent M1 for dilution of ram semen
  • as a component of Platz diluent variant (PDV) for dilution of bottlenose dolphin semen
  • in the preparation of HyQ PF-Chinese hamster ovary (CHO) disaccharide media for the adaptation of CHO cell lines
  • for the preparation of protein-free chemically defined cell culture medium (PFCDM) for the adaptation of human embryonic kidney 293 (HEK293) cells

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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A Thiel et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 125, 528-539 (2019-02-06)
3-NOP (3-nitrooxypropanol) reduces enteric methane formation in ruminants. A series of ADME studies in rats, lactating goats and beef cattle was performed. 3-NOP was entirely absorbed from the GIT of rats: approximately 75% of the administered 3-NOP was eliminated as
T R Robeck et al.
Biology of reproduction, 70(5), 1340-1348 (2004-01-09)
Research was conducted to develop an effective method for cryopreserving bottlenose dolphin (Tursiops truncatus) semen processed immediately after collection or after 24-h liquid storage. In each of two experiments, four ejaculates were collected from three males. In experiment 1, three
Ling Zhang et al.
Theranostics, 10(24), 11339-11358 (2020-10-13)
Background: TSTA3 gene encodes an enzyme responsible for synthesis of GDP-L-fucose as the only donor in fucosylation. This study was designed to explore clinical value, function and underlying mechanism of TSTA3 in the development of esophageal squamous cell carcinoma (ESCC).
Dawn Sow Zong Leong et al.
Scientific reports, 7, 45216-45216 (2017-03-31)
Mammalian cells are generally considered to be unable to utilize polysaccharides for cell growth because the phospholipid bilayer in the cell membrane has very low permeability to sugars. With the recent discovery of the only known animal disaccharide transporter, a
Johannes Gursch et al.
Journal of pharmaceutical sciences, 104(10), 3481-3489 (2015-07-07)
Over the last years, continuous manufacturing has created significant interest in the pharmaceutical industry. Continuous filtration at low flow rates and high solid loadings poses, however, a significant challenge. A commercially available, continuously operating, dynamic cross-flow filtration device (CFF) is
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