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Sigma-Aldrich

D-Lactic Dehydrogenase from Lactobacillus leichmannii

ammonium sulfate suspension, ≥250 units/mg protein (biuret)

Synonym(s):

Lactate, (R)-Lactate:NAD+ oxidoreductase, D−LDH

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About This Item

CAS Number:
9028-36-8
Enzyme Commission number:
1.1.1.28 (BRENDA, IUBMB)
MDL number:
MFCD00130907
UNSPSC Code:
12352204
NACRES:
NA.54

form

ammonium sulfate suspension

Quality Level

200

specific activity

≥250 units/mg protein (biuret)

foreign activity

Malic dehydrogenase <0.5% of base activity

storage temp.

2-8°C

General description

D-lactate dehydrogenase belongs to the D-isomer-specific 2-hydroxyacid dehydrogenase family.

Biochem/physiol Actions

D-lactic dehydrogenase enzyme is required for the production and metabolism of D-lactate. D-lactate is metabolized to pyruvate with the help of lactic dehydrogenase enzyme.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, pH 6.0

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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H Taguchi et al.
The Journal of biological chemistry, 266(19), 12588-12594 (1991-07-05)
The gene encoding D-lactate dehydrogenase (D-lactate: NAD+ oxidoreductase, EC 1.1.1.28) of Lactobacillus plantarum has been sequenced, and expressed in Escherichia coli cells with an inducible expression plasmid, in which the 5'-noncoding region of the gene was replaced with the tac
Torben Larsen
Analytical biochemistry, 539, 152-157 (2017-11-06)
D-lactic acid in the mammalian body is mainly of microbiological origin and is often located somewhere along the digestive tract. Surgical, extensive re-sectioning of the small bowel may be one of the risk factors for altered balance in the microbiological
C L McLaughlin et al.
Journal of dairy science, 92(6), 2758-2766 (2009-05-19)
A challenge model was used to evaluate a new approach to controlling acute acidosis. Acute acidosis reduces performance in both dairy and beef cattle and most often occurs as a consequence of ingestion of large amounts of readily fermentable starch
Zhaojuan Zheng et al.
Applied and environmental microbiology, 78(9), 3480-3483 (2012-02-22)
NAD-dependent l- and d-lactate dehydrogenases coexist in Lactobacillus genomes and may convert pyruvic acid into l-lactic acid and d-lactic acid, respectively. Our findings suggest that the relative catalytic efficiencies of ldhL- and ldhD-encoded products are crucial for the optical purity
Wenwen Zhao et al.
Analytical chemistry, 84(15), 6701-6706 (2012-07-04)
We proposed the first application of an electrophoretically mediated microanalysis (EMMA) method for fast online discrimination and determination of substrate enantiomers, which was achieved by just one EMMA assay. Lactate dehydrogenase (LDH)-catalyzed reaction was studied to evaluate the feasibility and
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