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L0302511

Sigma-Aldrich

ETT Activator

Synonym(s):

5-(Ethylthio)-1H-tetrazole, 5-(Ethylthio)tetrazole solution

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About This Item

MDL number:
MFCD00068733
UNSPSC Code:
12352200
NACRES:
NA.21

type

for DNA synthesis

Quality Level

200

product line

Proligo Reagents

packaging

pkg of 4 × 2.5 L L0302511-04

concentration

39-43 g/L (titration)

impurities

≤30 ppm water content (Karl Fischer)

color

clear

SMILES string

CCSc1nnn[nH]1

InChI

1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)

InChI key

GONFBOIJNUKKST-UHFFFAOYSA-N

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General description

ETT activator is an activator solution containing 5-(Ethylthio)-1H-tetrazole – a powerful activator – in acetonitrile. It is mixed with phosphoramidite during the extension step in which it reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.

Application

ETT activator has been used in the fast and low-cost synthesis of photolithographic DNA microarrays.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

35.6 °F

flash_point_c

2 °C

Certificates of Analysis (COA)

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Matej Sack et al.
Journal of nanobiotechnology, 14, 14-14 (2016-03-05)
DNA microarrays are a core element of modern genomics research and medical diagnostics, allowing the simple and simultaneous determination of the relative abundances of hundreds of thousands to millions of genomic DNA or RNA sequences in a sample. Photolithographic in

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