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H9269

Sigma-Aldrich

Hanks′ Balanced Salt solution

With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

HBSS

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About This Item

UNSPSC Code:
12352207

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 0.35 g/L
phenol red: 0.011 g/L
glucose: 1.0 g/L (Dextro)

shipped in

ambient

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General description

Formulation: click here.

Application

Hanks′ balanced salt solution has been used:
  • in starvation experiments for mammalian cells
  • to collect and maintain brain pieces for primary glial cell culture
  • to wash femoral tissue and bone marrow (BM) to culture mesenchymal stem cells

Biochem/physiol Actions

Balanced salt solution (BSS) in cell culture is multifaceted and has various functions. It serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. BSS provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It combines with carbohydrates, such as glucose to act as the principal energy source for cell metabolism. BSS also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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