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H0392

Sigma-Aldrich

Hemoglobin S, Ferrous Stabilized human

lyophilized powder

Synonym(s):

Sickle cell hemoglobin

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About This Item

CAS Number:
9035-22-7
MDL number:
MFCD00131288
UNSPSC Code:
12352202
eCl@ss:
42030116
NACRES:
NA.61

biological source

human

Quality Level

200

assay

97-100% (agarose gel electrophoresis)

form

lyophilized powder

technique(s)

electrophoresis: suitable
immunofluorescence: suitable

solubility

H2O: soluble 20 mg/mL

suitability

suitable for electrophoresis and chromatography standard (has not been tested for functional equivalence against native preparations (unlyophilized ferrous hemoglobins).)

UniProt accession no.

P68871

storage temp.

−20°C

Gene Information

human ... HBB(3043)

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General description

Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide.

Application

Hemoglobin S was used in the determination of fetal hemoglobin by time-resolved immunofluorometric assay.

Packaging

Package size indicates the amount of hemoglobin as determined by the procedure of Drabkin, D.L., J. Biol. Chem., 164, 703 (1946).

Reconstitution

When reconstituted with buffer, gives >90% ferrous hemoglobin.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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U Turpeinen et al.
Clinical chemistry, 38(10), 2013-2018 (1992-10-01)
We have developed a "sandwich"-type time-resolved immunofluorometric assay (IFMA) for fetal hemoglobin (HbF) in hemolysates from adults and newborns, amniotic fluid, and plasma, based on a polyclonal and a monoclonal antibody against human fetal hemoglobin. Microtiter wells are coated with
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Science advances, 5(3), eaau1086-eaau1086 (2019-03-21)
In sickle cell disease, the aberrant assembly of hemoglobin fibers induces changes in red blood cell morphology and stiffness, which leads to downstream symptoms of the disease. Therefore, understanding of this assembly process will be important for the treatment of
Charles T Quinn et al.
British journal of haematology, 175(4), 724-732 (2016-09-09)
Sickle cell disease (SCD) is a significant healthcare burden worldwide, but most affected individuals reside in low-resource areas where access to diagnostic testing may be limited. We developed and validated a rapid, inexpensive, disposable diagnostic test, the HemoTypeSC

Chromatograms

application for HPLC

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