Skip to Content
MilliporeSigma
All Photos(1)

Documents

GE17-5056-01

HiTrap® CM Fast Flow

Cytiva 17-5056-01, pack of 5 × 1 mL

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

carboxymethyl

description

Ion Exchanger Type (value)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-5056-01

parameter

42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14

working range

4-13

suitability

suitable for bioprocess medium

Related Categories

General description

HiTrap® CM FF is prepacked with CM Sepharose Fast Flow, and is a weak cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Application

Q, SP, DEAE adn CM Sepharose Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.

Features and Benefits

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High fLow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 8

1 of 8

HiTrap® SP Fast Flow Cytiva 17-5157-01, pack of 5 × 5 mL

GE17-5157-01

HiTrap® SP Fast Flow

HiTrap® Q Fast Flow Cytiva 17-5156-01, pack of 5 × 5 mL

GE17-5156-01

HiTrap® Q Fast Flow

MES ULTROL® Grade, ≥99% dry basis (titration), zwitterionic buffer useful in pH range 5.5-6.5

Millipore

475893

MES

HiTrap® IEX Selection Kit Cytiva 17-6002-33

GE17-6002-33

HiTrap® IEX Selection Kit

HiTrap® Q HP Cytiva 29-0513-25, pack of 1 mL

GE29-0513-25

HiTrap® Q HP

HisTrap™ Fast Flow Cytiva 17-5255-01, pack of 5 × 5 mL

GE17-5255-01

HisTrap Fast Flow

Bovine Serum Albumin lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

A9418

Bovine Serum Albumin

Articles

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service