GE17-0404-01
HiTrap® Protein G High Performance
Cytiva 17-0404-01, pack of 5 × 1 mL
About This Item
Recommended Products
ligand
recombinant protein G lacking albumin-binding region
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-0404-01
storage condition
(20% Ehtanol)
(20% Ethanol)
parameter
<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
size
avg. part. size
34 μm
cleaning
2-9(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-9(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity
>1 mg binding capacity (lgG/column)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
25 mg binding capacity(human IgG/column)
storage temp.
2-8°C
Related Categories
General description
Features and Benefits
- High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
- 34 μm bead size for high performance, high-resolution purifications.
- Binds broad range of different species and subclasses of IgG.
- Simple and proven method giving reproducible results.
- Designed for use with syringe, peristalic pump, or chromatography system.
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
Antibodies are needed for research and industrial purposes in different quantities, from microgram to kilogram scale. It is important to design and use a purification method that will yield protein of a quality and quantity that is adequate for the particular application.
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
Protocols
General instructions for affinity purification using HiTrap columns from Cytiva.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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