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G2580

Sigma-Aldrich

Sephadex® G-25

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About This Item

CAS Number:
9041-35-4
MDL number:
MFCD00132770
UNSPSC Code:
23151817
NACRES:
NA.56

Quality Level

200

technique(s)

buffer exchange: suitable

matrix active group

phase

swelling

1 g swells to 4-6 mL

bead size

20-80 μm

application(s)

life science and biopharma

compatibility

Cytiva

InChI

1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1

InChI key

BCPVZFFJGCURNR-OCOFDJSDSA-N

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General description

Sephadex® is a beaded gel filtration medium prepared by cross-linking dextran with epichlorohydrin under alkaline conditions. It is used in affinity chromatography, protein chromatography and gel filtration chromatography.
Fractionation Range (MW)
Globular Proteins: 1,000 - 5,000
Dextrans: 100 - 5,000

Application

Sephadex® G-25 has been used in size-exclusion chromatography to remove excess dye from labelled proteins.

Other Notes

G2580-100G′s updated product number is GE17-0032-01
G2580-500G′s updated product number is GE17-0032-02

Legal Information

Sephadex is a registered trademark of Cytiva

replaced by

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sephadex® G-25 Coarse Cytiva 17-0034-02, pack of 500 g

GE17-0034-02

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Sigma-Aldrich

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Celine Hoffmann et al.
Analytical biochemistry, 372(1), 62-71 (2007-09-11)
Fluorescein isothiocyanate (FITC) is a well-known probe for labeling biologically relevant proteins. However, the impact of the labeling procedure on protein structure and biological activities remains unclear. In this work, FITC-labeled human plasma fibronectin (Fn) was developed to gain insight
Jennifer Belyea et al.
Biochemistry, 45(48), 14275-14284 (2006-11-30)
The study of axial ligation by anionic ligands to ferric heme iron by resonance Raman spectroscopy provides a basis for comparison of the intrinsic electron donor ability of the proximal histidine in horse heart myoglobin (HHMb), dehaloperoxidase (DHP), and horseradish
Conformations of a metastable SH3 domain characterized by smFRET and an excluded-volume polymer model.
Mazouchi A, et al.
Biophysical Journal, 110(7), 1510-1522 (2016)
P M G Paiva et al.
Bioresource technology, 88(1), 75-79 (2003-02-08)
A highly purified trypsin inhibitor was obtained from Echinodorus paniculatus when an extract prepared from E. paniculatus seed flour (25 gl(-1), with 0.1 M ammonium acetate buffer, pH 8.3, under agitation for 6 min at 28 degrees C) was chromatographed
Zhifeng Huang et al.
Journal of biotechnology, 142(3-4), 242-249 (2009-05-30)
Recombinant human keratinocyte growth factor 2 (rhKGF-2) is a member of fibroblast growth factor protein family currently being investigated for its promising significant effects in treating epithelial damage. Molecular modification with polyethylene glycol (PEGylation) is an effective approach to improve
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