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F3651

Sigma-Aldrich

Fluorescein 5(6)-isothiocyanate

≥90% purity (HPLC), powder

Synonym(s):

FITC, Fluorescein isothiocyanate

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About This Item

Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
27072-45-3
Molecular Weight:
389.38
Beilstein/REAXYS Number:
1407295
EC Number:
248-207-7
MDL number:
MFCD00005063
UNSPSC Code:
12171500
PubChem Substance ID:
24894855
NACRES:
NA.47

product name

Fluorescein 5(6)-isothiocyanate, ≥90% (HPLC)

Quality Level

200

assay

≥90% (HPLC)

form

powder

color

yellow to orange

solubility

acetone: 1 mg/mL, clear, light yellow to yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

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General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

Fluorescein 5(6)-isothiocyanate has been used:
  • for fluorescence labeling of bacteria
  • as a standard for calibration of fluorometer
  • for the labeling of exosomes
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

wgk_germany

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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