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CY0100

Sigma-Aldrich

Cytochrome c Reductase (NADPH) Assay Kit

1 kit sufficient for 100 tests, determining cytochrome c reductase activity

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

200

usage

 kit sufficient for 100 tests

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... POR(5447)

Related Categories

Biochem/physiol Actions

Eukaroytic NADPH-cytochrome c reductase (NADPH-cytochrome P450 reductase, EC1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which are the cytochrome P450 family of enzymes, responsible for xenobiotic detoxification. NADPH-cytochrome c reductase is widely used as an endoplasmic reticulum marker and as one of the biomarkers for ecological pollution and dietary lipid uptake.

Features and Benefits

  • Detection of endoplasmic reticulum (ER) during isolation and subcellular fractionation by density gradient separation
  • Colorimetric measurement of NADPH-Cytochrome C Reductase activity in various sources after intoxication by drugs or challenge by xenobiotics
  • Analyze enrichment and function of ER

Analysis Note

The Cytochrome C Reductase Assay Kit contains all the reagents required for a fast and convenient determination of NADPH-Cytochrome C Reductase activity in cell and tissue extracts and in purified microsomes. It has been tested on samples prepared from various species of mammalian tissues such as liver, kidney, brain, spleen, and heart muscle, as well as on lysates from cell lines such as HeLa, HepG2, and Jurkat. In addition, it has been tested on samples prepared from the yeast strains P. pastoris and S. cerevisiae.

Kit Components Only

Product No.
Description
  • Assay Buffer 100 mL
  • Enzyme Dilution Buffer 25 mL
  • Cytochrome c 50 mg
  • Cytochrome c Reductase (NADPH) Positive Control from rabbit liver 50 μL
  • Cytochrome c Oxidase Inhibitor Solution 1 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mgSDS

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Product No.
Description
Pricing

pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 3

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

Certificates of Analysis (COA)

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Rohit Sharma et al.
Age (Dordrecht, Netherlands), 36(4), 9686-9686 (2014-07-20)
Imbalance in Th1/Th2 immune pathways and cellular antioxidant systems with progressive aging are among the leading causes of increased risk of morbidity and mortality in elderly. Although probiotics have been considered to boost immune system, there is a lack of
Nathan M Long et al.
Reproductive biology and endocrinology : RB&E, 11, 34-34 (2013-05-10)
Previously we reported decreased circulating progesterone and fertility in one and two year old ewes born to undernourished mothers. This study was designed to investigate if this reduction in progesterone persisted into old age, and if it did, what mechanisms
D Plonne et al.
Analytical biochemistry, 276(1), 88-96 (1999-12-10)
A novel method is described for the separation on a single gradient of the major intracellular organelles of the secretory pathway, the Golgi, the smooth endoplasmic reticulum, and the rough endoplasmic reticulum. Total microsomes were prepared from rat liver by
Amanda Spring de Almeida et al.
Life sciences, 276, 119469-119469 (2021-04-04)
Breast cancer-induced chronic pain is usually treated with opioids, but these compounds cause various adverse effects. Transient receptor potential ankyrin 1 (TRPA1) is involved in cancer pain; also, endogenous TRPA1 agonists are associated with cancer pain development. The aim of
C O Lemley et al.
Journal of dairy science, 93(3), 1012-1021 (2010-02-23)
In the cow, inadequate concentrations of progesterone during gestation may lead to an abrupt termination of pregnancy. The primary organ involved in progesterone catabolism is the liver, which contains an abundance of cytochrome P450 isozymes (EC 1.14.14.1; mixed-function monooxygenases). The
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