Huh-7D 12 Cell Line human
01042712
Synonym(s):
Huh7 Cells, JTC-39 Cells, Japanese Tissue Culture-39 Cells
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About This Item
UNSPSC Code:
41106514
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biological source
human liver
growth mode
Adherent
karyotype
That of original Huh-7
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
storage temp.
−196°C
Related Categories
General description
Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.
Cell Line Origin
Human hepatocellular carcinoma
Cell Line Description
Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.
Application
Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18
Culture Medium
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37 C. Media change twice a week
Other Notes
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