C-39270
Myocyte Growth Medium
SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml
Synonym(s):
Recommended with C-22270
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.81
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form
liquid
packaging
pkg of 1 kit
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Myocyte Growth Medium SupplementPack (basal medium sold separately)
To form a complete medium, it should be combined with the basal medium: C-22270
To form a complete medium, it should be combined with the basal medium: C-22270
Application
Myocyte Growth Medium is a low serum (5% V/V) medium supplemented with well chosen hormones and growth factors. It was developed for the optimal expansion and maintenance of primary cardiac myocytes in culture. The medium is optimized for primary human cells but can also be used for primary porcine cardiac myocytes.Recommended for:Human Cardiac Myocytes (HCM)
Components
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
Quality
All lots of PromoCell Myocyte Growth Medium are subjected to comprehensive quality control tests using primary human cardiac myocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested myocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Preparation Note
Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEpidermal Growth Factor (recombinant human): 0.5 ng / mlBasic Fibroblast Growth Factor (recombinant human): 2 ng / ml Insulin (recombinant human): 5 µg / ml
Storage and Stability
Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Subculture Routine
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Certificates of Analysis (COA)
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Articles
Primary human cardiac myocytes isolated from normal human ventricle tissue of the adult heart, plus optimized myocyte media. Protocols for handling, cell passaging, media, and product use.
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