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C-23020

Sigma-Aldrich

Fibroblast Growth Medium 2

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

Synonym(s):

Primary Cell Culture Media

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

liquid

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

General description

Fibroblast Growth Medium 2 (Ready-to-use)

Application

Fibroblast Growth Medium 2 is a low-serum medium and has been developed for the in vitro cultivation of adult Human Fibroblasts. The medium is optimized for primary human cells, but can also be used for bovine, porcine, and rat fibroblasts, as well as fibroblast cell lines.Recommended for:Normal Human Dermal Fibroblasts (NHDF), adult donorHuman Pulmonary Fibroblasts (HPF)Human Aortic Adventitial Fibroblasts (HAoAF)Human Uterine Fibroblasts (HUF)

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Fibroblast Growth Medium 2 are subjected to comprehensive quality control tests using primary human adult fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested adult fibroblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.02 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Primary Human Fibroblasts and Growth Media

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

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