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C-12360

Sigma-Aldrich

Human Pulmonary Fibroblasts (HPF)

500,000 cryopreserved cells

Synonym(s):

Human fibroblast culture

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human lung

packaging

pkg of 500,000 cells

morphology

(fibroblast)

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

pulmonary disorders

shipped in

dry ice

storage temp.

−196°C

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General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Lungs

Application

Primary Human Pulmonary Fibroblasts (HPF) are isolated from human lung tissue and are provided in a cryopreserved format. They are often used for studying lung tissue repair, tissue remodeling after injury, or the response after pulmonary inflammation. Fibroblast disorders like pulmonary fibrosis or hypoxia-induced pulmonary hypertension can also be investigated with cultured HPF.

Quality

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: CD90 positiveGuaranteed population doublings: >15

Recommended products

Recommended Primary Cell Culture Media:Link

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Articles

Primary Human Fibroblasts and Growth Media

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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