C0897
Citrate Lyase from Klebsiella pneumoniae
lyophilized powder, ≥0.20 unit/mg solid
Synonym(s):
Citrate oxaloacetate lyase
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About This Item
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form
lyophilized powder
Quality Level
specific activity
≥0.20 unit/mg solid
mol wt
>5 kDa
storage temp.
2-8°C
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Application
Citrate Lyase from Klebsiella pneumoniae has been used in the digestion of low molecular weight human milk fraction (>5 kDa referred to as 5kF).
Biochem/physiol Actions
Citrate lyase is found in several microorganisms and catalyzes the first step of citrate degradation, forming acetate and oxaloacetate. The enzyme contains 3 polypeptide subunits, α-subunit (a transferase), β-subunit (acyl lyase) and γ-subunit (acyl-carrier protein).
Unit Definition
One unit will convert 1.0 μmole of citrate to oxalacetate per min at pH 7.6 at 25 °C.
Physical form
Lyophilized powder containing bovine serum albumin, sucrose, and MgSO4
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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S Bekal-Si Ali et al.
Journal of bacteriology, 181(14), 4411-4416 (1999-07-10)
In this paper, we describe two open reading frames coding for a NAD-dependent malic enzyme (mae) and a putative regulatory protein (clyR) found in the upstream region of citCDEFG of Leuconostoc mesenteroides subsp. cremoris 195. The transcriptional analysis of the
Celia W Goulding et al.
Journal of molecular biology, 365(2), 275-283 (2006-10-27)
Fatty acid biosynthesis is essential for the survival of Mycobacterium tuberculosis and acetyl-coenzyme A (acetyl-CoA) is an essential precursor in this pathway. We have determined the 3-D crystal structure of M. tuberculosis citrate lyase beta-subunit (CitE), which as annotated should
R Singh et al.
Journal of biochemical and biophysical methods, 64(3), 189-199 (2005-09-13)
We demonstrate a facile blue native polyacrylamide gel electrophoresis (BN-PAGE) technique to detect two malate-generating enzymes, namely fumarase (FUM), malate synthase (MS) and four oxaloacetate-forming enzymes, namely pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase (PEPCK), citrate lyase (CL) and aspartate aminotransferase (AST).
M Martín et al.
Journal of bacteriology, 182(14), 3904-3912 (2000-06-27)
In this study we describe the expression pattern of the Leuconostoc paramesenteroides citMCDEFGRP operon in response to the addition of citrate to the growth medium. An 8.8-kb polycistronic transcript, which includes the citMCDEFGRP genes, was identified; its synthesis was dramatically
M Isabel Burguete et al.
Dalton transactions (Cambridge, England : 2003), (36)(36), 4027-4033 (2007-09-11)
A turn-on fluorescent indicator for citric acid (citrate) has been developed, displaying high emission enhancement (+1500%) and low interference by other carboxylates. The sensor is based on the non-emissive copper(II) complex of a fluorescent amino amide, which, upon addition of
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