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B9879

Sigma-Aldrich

BES

≥99.0% (titration)

Synonym(s):

2-(bis(2-hydroxyethyl)amino)ethane sulfonic acid, n,n-bis[2-hydroxyethyl]-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine

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About This Item

Empirical Formula (Hill Notation):
C6H15NO5S
CAS Number:
10191-18-1
Molecular Weight:
213.25
Beilstein/REAXYS Number:
1781572
EC Number:
233-465-5
MDL number:
MFCD00007533
UNSPSC Code:
12161700
PubChem Substance ID:
24892162
NACRES:
NA.25

Quality Level

400

assay

≥99.0% (titration)

form

crystalline

storage condition

dry at room temperature

color

white

pH

2.5-5.0 (25 °C, 213.3 g/L)

useful pH range

6.4-7.8

pKa (25 °C)

7.1

solubility

water: 0.5 g/mL, clear, colorless

density

1.30 g/cm3 at 20—25 °C
1.35 g/cm3 at 20—25 °C

application(s)

clinical research
diagnostic assay manufacturing
general analytical
life science and biopharma

storage temp.

room temp

SMILES string

OCCN(CCO)CCS(O)(=O)=O

InChI

1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)

InChI key

AJTVSSFTXWNIRG-UHFFFAOYSA-N

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General description

N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, known as BES, is a zwitterionic buffer and a valuable secondary standard in biochemical research. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes. The useful pH range for BES buffer is 6.4 to 7.8. It is one of the "Good" buffers developed in the 1960s to provide buffering capacity in the pH range of 6.15 to 8.35, making it widely applicable in biochemical studies. BES buffer is suitable for use in systems where the formation of a calcium phosphate-DNA complex is desirable (Absorbance: < 0.05 at 260 nm, 100 mM). Further, BES buffer can be employed in the study of molecular biology, biological buffers, molecular biology reagents, and zwitterionic compounds.

Application

BES may be employed as binding buffer in modified Eagle′s medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.
Further, BES has found application in transfection studies, both in the preparation of reagents for transfection and in signaling assays related to Neural Melanocortin Receptors.

Features and Benefits

  • Suitable for Cell Culture media and as a Buffering solution in Biochemical and Molecular Biology Research
  • Effective Buffering from pH 2.5-5.0 (25 °C, 213.3 g/L) with a pKa of 7.1 (25 °C)
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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Certificates of Analysis (COA)

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Second-stage dissociation of N,N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (?bes?) in water and in 50 mass per cent methanol+ water from 278.15 to 328.15 K.
Roy RN, et al.
The Journal of Chemical Thermodynamics, 9(4), 325-332 (1977)
Water sorption, proton conduction, and methanol permeation properties of sulfonated polyimide membranes cross-linked with N, N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (BES).
Lee CH, et al.
Macromolecules, 39(2), 755-764 (2006)
Marina V Kirillova et al.
Inorganic chemistry, 51(9), 5224-5234 (2012-04-10)
The facile aqueous medium reactions of copper(II) nitrate with BES biobuffer [(HOCH(2)CH(2))(2)N(CH(2)CH(2)SO(3)H), hereinafter referred as H(3)bes] in the presence of various benzenecarboxylic acids [benzoic (Hba), 3-hydroxybenzoic (Hhba), and 3,5-dihydroxybenzoic (Hdhba) acid] and lithium hydroxide gave rise to the self-assembly generation
R N Fabricant et al.
Proceedings of the National Academy of Sciences of the United States of America, 74(2), 565-569 (1977-02-01)
Purified mouse nerve growth factor (NGF)radiolabeled with 125I was tested for its ability to bind to a variety of different cultured cells. NGF binds readily to human and hamster melanoma cells but does not bind to many other cell lines.
John A Ormiston et al.
EuroIntervention : journal of EuroPCR in collaboration with the Working Group on Interventional Cardiology of the European Society of Cardiology, 10(12), 1449-1455 (2014-11-26)
Stent fracture is important because it may cause adverse events. The interventional cardiologist needs independent data to aid stent selection. Stent designers need data to improve stent design. We used a repetitive bend test to compare durability and fracture for
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