AP0100
Alkaline Phosphatase Diethanolamine Activity Kit
sufficient for 100 tests
Synonym(s):
ALP Activity Kit
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.26
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General description
The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.
Application
Alkaline Phosphatase Diethanolamine Activity Kit has been used:
- in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblasts
- for monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cells
- for determining ALP activity in the bone metastasis model of lung cancer
- for measuring ALP levels in colon tissue homogenates
Suitability
Suitable for the measurement of alkaline phosphatase activity in a variety of samples
Principle
Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 C, the reaction is followed by monitoring the increase in absorbance at 405 nm.
signalword
Danger
Hazard Classifications
Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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