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A8452

Sigma-Aldrich

Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse

ascites fluid, clone C3

Synonym(s):

Afp Antibody, Afp Antibody - Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse, Alpha Fetoprotein Antibody

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About This Item

MDL number:
MFCD00162111
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

C3, monoclonal

contains

15 mM sodium azide

species reactivity

pig, canine, human

should not react with

mouse, cat, rat, bovine

technique(s)

dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human fetal liver
indirect ELISA: suitable

isotype

IgG2a

UniProt accession no.

P02771

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AFP(174)

General description

α-Fetoprotein (AFP) is a one of the main glycoprotein present in the fetal liver, gastrointestinal tract, and yolk sac. AFP levels in amniotic fluid have been used as biomarkers for anencephaly and spina bifida.
Monoclonal Anti-α-Fetoprotein (AFP) (mouse IgG2a isotype) is derived from the C3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human α-fetoprotein.

Immunogen

human α-fetoprotein

Application

Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • dot blot
  • immunocytochemistry
  • immunofluorescence
  • immunocytochemistry

Mouse Monoclonal Anti-α-Fetoprotein has been used for immunocytochemistry. The product can also be used for dot blot and indirect ELISA.

Biochem/physiol Actions

Increased concentrations of α-fetoprotein (AFP) in serum are less common in patients with malignancies of the gastrointestinal tract and of other organ systems with massive hepatic metastases. Hence, AFP determination is useful for the diagnosis of certain germ line tumors that contain yolk sac structures (testicular and ovarian tumors), hepatocellular carcinoma (hepatoma), and some malignant tumors of the gastrointestinal tract. AFP serves as an important marker for certain genetic and embryonic defects. Routine screening of pregnancies with a risk of neural tube defects (spina bifida, anencephaly, and Down′s syndrome) may be facilitated by the monitoring of AFP levels.

Target description

AFP is a plasma protein found highly expressed in the human fetus where it functions to block the transport of estradiol across the placenta.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Alpha-fetoprotein in the antenatal diagnosis of anencephaly and spina bifida.
D J Brock et al.
Lancet (London, England), 2(7770), 197-199 (1972-07-29)
Alpha Fetoprotein (AFP, Maternal Serum Alpha Fetoprotein, MSAFP)
NCBI Bookshelf (2019)
Reduced PLP1 expression in induced pluripotent stem cells derived from a Pelizaeus-Merzbacher disease patient with a partial PLP1 duplication
Shimojima K, et al.
Journal of Human Genetics, 57(9), 580-580 (2012)
Anne M Bara et al.
Stem cell research, 17(2), 441-443 (2016-11-24)
Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein
Characterization of a novel embryonic stem cell line from an ICSI-derived blastocyst in the African green monkey
Shimozawa N, et al.
Reproduction (Cambridge, England), 139(3), 565-565 (2010)
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