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A2559

Sigma-Aldrich

N-Acetyl-Asp-Glu-Val-Asp p-nitroanilide

≥97% (HPLC), powder

Synonym(s):

Ac-DEVD-pNA

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About This Item

Empirical Formula (Hill Notation):
C26H34N6O13
CAS Number:
189950-66-1
Molecular Weight:
638.58
MDL number:
MFCD00792707
UNSPSC Code:
12352209
PubChem Substance ID:
24890668
NACRES:
NA.32

Quality Level

100

assay

≥97% (HPLC)

form

powder

color

white

storage temp.

−20°C

SMILES string

CC(C)[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(C)=O)C(=O)N[C@@H](CC(O)=O)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C26H34N6O13/c1-12(2)22(26(43)30-18(11-21(38)39)24(41)28-14-4-6-15(7-5-14)32(44)45)31-23(40)16(8-9-19(34)35)29-25(42)17(10-20(36)37)27-13(3)33/h4-7,12,16-18,22H,8-11H2,1-3H3,(H,27,33)(H,28,41)(H,29,42)(H,30,43)(H,31,40)(H,34,35)(H,36,37)(H,38,39)/t16-,17-,18-,22-/m0/s1

InChI key

GGXRLUDNGFFUKI-ORGXJRBJSA-N

Gene Information

human ... CASP3(836)

Amino Acid Sequence

NAc-Asp-Glu-Val-Asp-pNA

Application

N-Acetyl-Asp-Glu-Val-Asp p-nitroanilide has been used as a chromogenic substrates for determining caspase-3 and caspase-8 activities in liver tissue homogenates and caspase 3 activity in aortic vascular smooth muscle cells and neuroblastoma cell lines.

Biochem/physiol Actions

Colorimetric substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase.

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Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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