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Sigma-Aldrich

Immersion oil

for microscopy

Synonym(s):

immersion medium for light microscopy

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About This Item

MDL number:
MFCD00131369
UNSPSC Code:
12352200

biological source

synthetic

Quality Level

100

grade

for microscopy

form

liquid

refractive index

n20/D 1.516

viscosity

100-120 mPa.s(20 °C)

density

1.025 g/mL at 20 °C

application(s)

hematology
histology

storage temp.

room temp

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General description

Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to approximate the refractive index of glass as closely as possible. It is used in conjunction with an objective lens to enhance the resolving power.

Application

Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, hematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted.

Principle

Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

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Total internal reflection fluorescence (TIRF) microscopy uses the evanescent field on the aqueous side of a glass/aqueous interface to selectively illuminate fluorophores within approximately 100 nm of the interface. Applications of the method include epi-illumination TIRF, where the exciting light

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