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Sigma-Aldrich

5-Carboxy-tetramethylrhodamine N-succinimidyl ester

BioReagent, suitable for fluorescence

Synonym(s):

"4-Carboxytetramethylrhodamine N-succinimidyl ester", 5-TAMRA

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About This Item

Empirical Formula (Hill Notation):
C29H25N3O7
CAS Number:
Molecular Weight:
527.52
MDL number:
UNSPSC Code:
12352116
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Quality Level

solubility

DMF: soluble
acetonitrile: soluble

fluorescence

λex 543 nm; λem 578 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CN(C)c1ccc2c(OC3=C\C(C=CC3=C2c4ccc(cc4C([O-])=O)C(=O)ON5C(=O)CCC5=O)=[N+](\C)C)c1

InChI

1S/C29H25N3O7/c1-30(2)17-6-9-20-23(14-17)38-24-15-18(31(3)4)7-10-21(24)27(20)19-8-5-16(13-22(19)28(35)36)29(37)39-32-25(33)11-12-26(32)34/h5-10,13-15H,11-12H2,1-4H3

InChI key

VWFRSNKRTNUMET-UHFFFAOYSA-N

Application

5-Carboxy-tetramethylrhodamine N-succinimidyl ester (5-TAMRA) is used as an amine coupling reagent to form 5-carboxy-tetramethylrhodamine (5-TAMRA) derivatized compounds. 5-TAMRA derivatized compounds may be used in applications as enzyme substrates, molecular probes, molecular beacons and as fluorescence resonance energy transfer (FRET) partners.

Other Notes

Fluorescent labeling of DNA and RNA

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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