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O1875

Sigma-Aldrich

Orcinol monohydrate

colorimetric detection reagent

Synonym(s):

3,5-Dihydroxytoluene, 5-Methylresorcinol

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About This Item

Linear Formula:
CH3C6H3-1,3-(OH)2 · H2O
CAS Number:
6153-39-5
Molecular Weight:
142.15
Beilstein/REAXYS Number:
1071903
EC Number:
207-984-2
MDL number:
MFCD00149092
UNSPSC Code:
12171500
PubChem Substance ID:
24897953
NACRES:
NA.47

biological source

synthetic

Quality Level

200

assay

≥99% (HPLC)

form

powder

color

white to light brown

bp

290 °C (lit.)

mp

56-58 °C (lit.)

solubility

H2O: 50 mg/mL

density

1.290 g/cm3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

O.Cc1cc(O)cc(O)c1

InChI

1S/C7H8O2.H2O/c1-5-2-6(8)4-7(9)3-5;/h2-4,8-9H,1H3;1H2

InChI key

NBKPNAMTHBIMLA-UHFFFAOYSA-N

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General description

Orcinol is a small-molecule natural phenol involved in the production of a dye called orcein used for the staining of cells and chromosomes. Orcinol monohydrate is usually used to prepare a colorimetric reagent known as Bial′s reagent for the determination of RNA, DNA, and hexoses.

Application

Orcinol monohydrate has been used to prepare Bial′s reagent for Bial′s orcinol assay for pentose sugars. It is also used in the preparation of orcinol solution suitable for staining TLC plates and, measuring rhamnolipids in orcinol dye assays. Orcinol monohydrate has been used for the RNA assay.



pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Lipopolysaccharide binding protein expression in primary human hepatocytes and HepG2 hepatoma cells.
Grube BJ
The Journal of Biological Chemistry, 269, 8477-8477 (1994)
A simultaneous estimation method of DNA and RNA by the orcinol reaction and a study on the reaction mechanism.
Y Endo
Journal of biochemistry, 67(5), 629-633 (1970-05-01)
Marjan De Mey et al.
Analytical biochemistry, 353(2), 198-203 (2006-03-21)
Recent developments in cellular and molecular biology require the accurate quantification of DNA and RNA in large numbers of samples at a sensitivity that enables determination on small quantities. In this study, five current methods for nucleic acid quantification were
A modified orcinol reaction for RNA determination.
M Kamali et al.
Clinical chemistry, 15(5), 390-392 (1969-05-01)
Estimation of monosaccharides by the orcinol-sulphuric acid reaction.
J BRUCKNER
The Biochemical journal, 60(2), 200-205 (1955-06-01)
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