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B3883

Millipore

Bile bovine

dried, unfractionated

Synonym(s):

Ox gall powder

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106212
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

dried, unfractionated

color

yellow-brown

solubility

water: soluble 50 mg/mL

application(s)

microbiology

General description

Bile bovine is extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media as a selective inhibitory agent for isolation and identification of pathogens. Bile is a yellow-green aqueous solution consisting of 70% bile salts, 22% phospholipids, 4% cholesterol, 3% proteins and 0.3% bilirubin. It is produced from cholesterol in liver hepatocytes and stored in the gallbladder. Bile acids are steroid acids found mainly in the bile of mammals and other vertebrates. They can be used as a selective agent in media for the detection of intestinal bacteria.

Application

Bile bovine has been used:
  • in De Man, Rogosa and Sharpe agar (MRS) broth for the growth test under high bile-salt condition
  • as a component in MRS to grow Lactobacillus delbrueckii subsp. lactis 200+,to study the effect of bile salts on cell morphology
  • as a component in brain heart infusion (BHI) broth for broth assays to study its effects on Enterococcus faecalis V583

Biochem/physiol Actions

Bile acids plays a physiological role in the removal of cholesterol from the body. It also participates in the intestinal solubilization and absorption of lipids.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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