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Agar

high purity, low ionic content, low gel strenght, suitable for microbiology

Synonym(s):

Agar-agar, Gum agar, Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106212
NACRES:
NA.85

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

ign. residue

≤4.0%

loss

≤10% loss on drying

turbidity

≤8 NTU (after autoclaving)

mp

85-95 °C

transition temp

gel point 34-36 °C

gel strength

≥800 g/cm2

anion traces

sulfate (SO42-): ≤2.75%

cation traces

Ca: ≤500 mg/kg
Mg: ≤200 mg/kg

application(s)

microbiology

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Agar is of high purity, low ionic content, and low gel strength widely used in microbiology as a solidifying agent in microbial culture media. It is a polysaccharide derived from seaweed and is primarily composed of agarose. Agar is a mostly inert substance, which makes it an ideal material for microbial culture media because it doesn’t interfere with the growth of microorganisms. Agar can solidify media, allowing microorganisms to grow in a solid environment, which facilitates the formation of distinct colonies and selective isolation of specific microorganisms. Agar is also used in the preparation of selective and differential media, which can provide additional nutrients or other chemical substances to promote or inhibit the growth of specific microorganisms selectively. Agar is sterilized by autoclaving and then poured into Petri dishes, where it can cool and solidify, forming the surface for microbial growth.

Application

Agar is commonly used as a solidifying agent in many microbial culture media for the growth and identification of bacteria, yeasts and molds from various pharmaceutical, environmental, food and beverage samples.

Analysis Note

toxic substances: absence

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Arnaud Dechesne et al.
Applied and environmental microbiology, 74(16), 5195-5200 (2008-07-01)
Water is arguably the most important constituent of microbial microhabitats due to its control of physical and physiological processes critical to microbial activity. In natural environments, bacteria often live on unsaturated surfaces, in thin (micrometric) liquid films. Nevertheless, no experimental
Queeny Wing-Han Yuen et al.
The open biomedical engineering journal, 5, 39-46 (2011-06-07)
Cervical lymph nodes are common sites of metastatic involvement in head and neck cancers. These lymph nodes are superficially located and palpation is a common practice for assessing nodal hardness and staging cancer which is, however, too subjective and with
GunNam Na et al.
The Journal of biological chemistry, 286(47), 40423-40432 (2011-09-21)
When growing in its native habitat, Thlaspi goesingense can hyperaccumulate 1.2% of its shoot dry weight as nickel. We reported previously that both constitutively elevated activity of serine acetyltransferase (SAT) and concentration of glutathione (GSH) are involved in the ability
E Reyssat et al.
Physical review letters, 109(24), 244301-244301 (2013-02-02)
A soft solid is more easily sliced using a combination of normal and shearing deformations rather than diced by squeezing down on it normally with the same knife. To explain why this is so, we experimentally probe the slicing and
Extracellular bioluminescence of mycelium metabolites of the luminous mushroom Panellus stipticus (IBSO-2301) growing on an Agar medium.
A P Puzyr et al.
Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 448, 39-40 (2013-03-13)

Articles

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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