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modified Tryptic Soy Agar- Dehydrated Culture Media

NutriSelect® Plus, lecithin, polysorbate 80 (Tween® 80), powder, suitable for microbiology

Synonym(s):

CASO Lecithin Polysorbate Agar, CASO-Lecithin-Polysorbat-Agar, Microbial Content Test Agar, Soybean Casein digest Agar with Polysorbate 80 and Lecithin, TSA Lecithin Polysorbate Agar, Tryptone Soya Lecithin Polysorbate Agar

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
lecithin
papaic digest of soyabean meal, 5 g/L
peptone from casein, 15 g/L
polysorbate 80 (Tween® 80)
sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

7.3±0.2 (25 °C)

application(s)

cleaning products
clinical testing
cosmetics
environmental
food and beverages
personal care
pharmaceutical
sterility testing
surface monitoring
microbiology

suitability

Micrococcus spp.
nonselective for Aspergillus spp.
nonselective for Bacillus spp.
nonselective for Candida spp.
nonselective for Enterococcus spp.
nonselective for Escherichia coli
nonselective for Pseudomonas spp.
nonselective for Salmonella spp.
nonselective for Serratia spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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General description

Tryptone Soya Agar with Polysorbate 80 and Lecithin is used for determining the efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics, etc. Tryptone and soya peptone provide nitrogenous and other nutrients required for the replication of microbes. Lecithin and Polysorbate 80 neutralize many residual disinfectants. Polysorbate 80 inactivate phenols, hexachlorophene and formalin, while lecithin neutralizes quaternary ammonium compounds.

Application

Tryptone Soya Agar with Polysorbate 80 and Lecithin is used to identify and quantify viable microbial contaminants present on surfaces of sanitary importance

Preparation Note

Suspend 45.7 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 12 to 15 lbs pressure (118°C - 121°C) for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Lot/Batch Number

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CASO Agar NutriSelect® Plus, powder, suitable for microbiology

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Sung-Chih Hsieh et al.
Materials (Basel, Switzerland), 13(2) (2020-01-16)
The objective of this study was to evaluate the antibacterial efficacy against Enterococcus faecalis and Streptococcus mutans and in vivo toxicity using embryonic zebrafish assays of sodium hypochlorite (NaOCl) and electrolyzed oxidizing (EO) water (containing hypochlorous acid (HOCl))-based root canal
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