EHIFIP-RO
Roche
Expand™ High FidelityPLUS PCR System
sufficient for ≤500 reactions (03300226001), sufficient for ≤2,500 reactions (03300234001), suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included: no
Synonym(s):
high fidelity pcr plus system, expand
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About This Item
Recommended Products
usage
sufficient for ≤2,500 reactions (03300234001)
sufficient for 2500 reactions
sufficient for ≤500 reactions (03300226001)
sufficient for 500 reactions
Quality Level
feature
High Fidelity PCR
dNTPs included: no
hotstart: no
packaging
pkg of 2,500 U (03300234001 [10x250 U])
pkg of 500 U (03300226001 [2x250 U])
manufacturer/tradename
Roche
parameter
72 °C optimum reaction temp.
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
Related Categories
General description
The Expand High FidelityPLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity.
The Expand High FidelityPLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High FidelityPLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.
The Expand High FidelityPLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High FidelityPLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.
Application
The Expand™ High FidelityPLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay.
Use Expand High FidelityPLUS PCR System for:
Use Expand High FidelityPLUS PCR System for:
- PCR
- RT-PCR
- Primer extension
- Carryover prevention
Features and Benefits
- Safeguard against cross-contamination.
- Obtain sixfold higher fidelity and higher yields,
- Efficiently label DNA.
Packaging
1 kit containing 4 components
Quality
Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.
Unit Definition
Volume Activity: 5 U/μL
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
Transition-metal-catalyzed cross-coupling is an efficient tool for constructing C-C, C-N and C-O bonds in organic synthesis. Intensive interest has been focused on the development of the most effective ligands that improve catalyst performance. Ligands play a key role in stabilizing and activating the central metal atom and fine-tuning the selectivity of transformation. Phosphines have proven to be the most significant class of ligands for cross couplings, such as Negishi, Suzuki, Stille, Heck, Sonogashira, and Buchwald-Hartwig cross-coupling.
Expand is a trademark of Roche
Kit Components Only
Product No.
Description
- Expand High FidelityPLUS Enzyme Blend 5 U/μl
- Expand High FidelityPLUS Reaction Buffer, with 7.5 mM MgCl2 5x concentrated
- Expand High FidelityPLUS Reaction Buffer, without MgCl2 5x concentrated
- MgCl2 Stock Solution 25 mM
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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