OVCAR-4 Human Ovarian Cancer Cell Line

Ovarian carcinoma is a prevalent worldwide disease, with over 200,000 new cases diagnosed per year (1). The first-line chemotherapy for newly-diagnosed ovarian carcinoma is cisplatin, but despite high initial efficacy, the most common types of ovarian carcinomas frequently relapse into platinum-resistant forms and many demonstrate multi-drug resistance (1). The availability of cellular models that recapitulate both resistant and susceptible forms of the disease are essential to understanding drug resistance and advancing new options for treatment.

OVCAR-4 is a high-grade serous ovarian adenocarcinoma cell line established from a patient refractory to cisplatin, and is resistant to multiple chemotherapeutic agents (2). OVCAR-4 cells are characterized by migration/invasion ability and are tumorigenic in nude mice (3). The OVCAR-4 cell line is homozygous for the Leu130Val mutation in TP53 (4) and expresses high levels of the cancer marker integrin αvβ3 (5). OVCAR-4 cells are recommended to be passaged at relatively high density (at least 13,000 cells/cm2). OVCAR-4 is one of the most representative cell lines for high-grade serous ovarian carcinoma and a highly relevant model for multidrug-resistant ovarian cancer.

Source:
OVCAR-4 was established from ascites from a 42-year-old patient with an ovarian tumor refractory to cisplatin treatment (2).

References:
1. Nat Rev Disease Primers 2016; 2: 16061.
2. Cancer Res 1997; 57: 850—856.
3. Gynecol Oncol 2015;138(2): 372-377.
4. Mol Cancer Ther 2006; 5(11): 2606-2612.
5. Bioorganic & Medicinal Chem 2018; 26: 2085-2091.

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.