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FCMAB104A4

Sigma-Aldrich

Anti-phospho H3 (Ser10) Antibody, clone 3H10 Alexa Fluor™ 488

clone 3H10, from mouse, ALEXA FLUOR™ 488

Synonym(s):

H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3H10, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG1κ

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

phosphorylation (pSer10)

Gene Information

human ... HIST1H3F(8968)

Related Categories

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Broad species cross-reactivity is expected.
Histone H3 phosphorylated at Ser10

Immunogen

A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3.
Epitope: Ser10

Application

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor™ 488, is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as H3S10p,
is labeled with Alexa Fluor 488 and validated for use in FC.
Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer
Research Sub Category
Histones

Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Flow Cytometry with Jurkat cells.

Target description

17 kDa observed

Physical form

Protein G Purified
Purified mouse IgG1k conjugated to Alexa Fluor™ 488 in PBS with 15mg/mL BSA and less than 0.09% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Analysis Note

Control
Jurkat Cells

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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