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ABE1407

Sigma-Aldrich

Anti-Ubiquitin-conjugating enzyme E2 A Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Ubiquitin-conjugating enzyme E2 A, RAD6 homolog A, HR6A, hHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human

species reactivity (predicted by homology)

primate (based on 100% sequence homology), sheep (based on 100% sequence homology), horse (based on 100% sequence homology), chicken (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_003327

UniProt accession no.

P49459

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UBE2A(7319)

General description

Ubiquitin-conjugating enzyme E2 A (EC 6.3.2.19; UniProt P49459; also known as hHR6A, HR6A, RAD6 homolog A, Ubiquitin carrier protein A) is encoded by the UBE2A (also known as MRXS30, MRXSN, RAD6A, UBC2) gene (Gene ID 7319) in human. UBE2A accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2A is involved in transcription regulation by catalyzing the monoubiquitination of histone H2B Lys120 to form H2BK120ub1. H2BK120ub1 serves as a specific signal for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, as well as a prerequisite for H3K4me and H3K79me formation. In vitro, UBE2A catalyzes both Lys11 and Lys48-linked polyubiquitination. UBE2A is also involved in UV-damaged DNA repair and aberrant UBE2A can cause mental retardation syndromic X-linked Nascimento-type (MRXSN).

Specificity

Expected to react with all three spliced isoforms.

Immunogen

Epitope: Near C-terminus
KLH-conjugated linear peptide corresponding to human Ubiquitin-conjugating enzyme E2 A sequence near the C-terminus.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-Ubiquitin-conjugating enzyme E2 A Antibody is validated for use in Western Blotting for the detection of Ubiquitin-conjugating enzyme E2 A.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ubiquitin-conjugating enzyme E2 A in 10 µg of HeLa cell lysate.

Target description

~17 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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