Anti-Nitric Oxide Synthase II Antibody

Nitric Oxide Synthases are the only enzymes know to simultaneously require five bound cofactors, FAD, FMN, haem, tetrahydrobiopterin (BH4) and Ca++-Calmodulin (CAM). In mammals, there are three distinct genes for NOS, NOS I is neuronal, NOS II is cytokine inducible, and NOS III is endothelial. The enzymes exist as homodimers, each monomer consisting of two major domains: N­terminal oxygenase domain, which belongs to the class of haem-thiolate proteins, and C­terminal reductase domain, which is homologous to NADPH:P450 reductase. The interdomain linker between the oxygenase and reductase domains contains a CaM­binding sequence. Functionally NOS is an enzyme that catalyzes the stepwise conversion of the amino acid L-arginine to nitric oxide and L-citrulline. NOS activity stimulates cyclic GMP, inducing smooth muscle relaxation by increasing intracellular cGMP, which in turn inhibits calcium entry into the cell, and decreases intracellular calcium concentrations. This also stimulates a cGMP-dependent protein kinase that activates myosin light chain phosphatase, the enzyme that dephosphorylates myosin light chains, which leads to smooth muscle relaxation.

Inducible nitric oxide synthase (iNOS, NOS-II). No cross-reaction with rat nNOS and eNOS by Western blot and immunohistochemistry.

Reactivity to human iNOS is approximately 1%.

C-terminal 14 amino acid peptide from mouse macrophage nitric oxide synthase.

Epitope: C-terminus

Anti-Nitric Oxide Synthase II Antibody is an antibody against Nitric Oxide Synthase II for use in IC, IH & WB.

Immunohistochemistry:
1:5000 (ABC) dilution of a previous lot was used on tissue sections (rat liver).

Immunocytochemistry:
1:50,000 dilution of a previous lot was used on cultured cells.

Western blot:
1:5,000 (ECL). The antibody has been used successfully on rat liver. It is not recommended for use on rat brain extracts since unidentified bands may be present.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

Evaluated by Western Blot on Mouse liver lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected INOS on 10 µg of Mouse liver lysates.

131 kDa

Rabbit polyclonal serum in buffer containing liquid 0.05% sodium azide.

Unpurified

Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Macrophages, liver lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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