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AB1766

Sigma-Aldrich

Anti-Dopamine Transporter Antibody, CT

Chemicon®, from rabbit

Synonym(s):

DAT

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

monkey, human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_001044.3

UniProt accession no.

Q01959

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SLC6A3(6531)

Specificity

Dopamine Transporter (DAT), C-terminal.

Immunogen

Synthetic peptide from the intracellular C-terminal region of human dopamine transporter (CEKDRELVDRGEVRQFTLRHWL).

Application

Anti-Dopamine Transporter Antibody, C-terminus detects level of Dopamine Transporter & has been published & validated for use in IH & WB.
Immunoblotting: 0.1-1.0 μg/mL. The antibody recognizes the ~88 kDa DAT protein in Western blot of human strial samples.
Immunohistochemistry using formaldehyde-fixed brain sections.
Not recommended for use on rodent.
Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in 100 μL of 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Storage and Stability

Maintain at -20 to -80°C in undiluted aliquots for up to six months after date of receipt. Avoid repeated freeze-thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cloning of dopamine, norepinephrine and serotonin transporters from monkey brain: relevance to cocaine sensitivity.
G M Miller, S M Yatin, R De La Garza, M Goulet, B K Madras, G M Miller, S M Yatin et al.
Brain Research Molecular Brain Research null
Zuzanna Kurowska et al.
Journal of Parkinson's disease, 1(1), 83-92 (2011-01-01)
We demonstrate that grafted human fetal mesencephalic neurons can survive and extend axons for 22 years in the brain of a patient with Parkinson's disease (PD). In this patient, the overall survival and fiber outgrowth of the grafts were, however
Chiharu Sogawa et al.
PloS one, 5(8), e11945-e11945 (2010-08-12)
The transporters for dopamine (DAT) and norepinephrine (NET) are members of the Na+- and Cl--dependent neurotransmitter transporter family SLC6. There is a line of evidence that alternative splicing results in several isoforms of neurotransmitter transporters including NET. However, its relevance
Patricia Marques Pereira et al.
Journal of neurochemistry, 107(5), 1325-1334 (2008-10-01)
The Coffin-Lowry syndrome, a rare syndromic form of X-linked mental retardation, is caused by loss-of-function mutations in the hRSK2 (RPS6KA3) gene. To further investigate RSK2 (90-kDa ribosomal S6 kinase) implication in cognitive processes, a mrsk2_KO mouse has previously been generated

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