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AB15322

Sigma-Aldrich

Anti-Nicotinic Acetylcholine Receptor α4 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Cholinergic receptor, nicotinic, alpha 4 (neuronal), Cholinergic receptor, nicotinic, alpha polypeptide 4, Neuronal nicotinic acetylcholine receptor alpha-4 subunit, Neuronal acetylcholine receptor subunit alpha-4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat, mouse

technique(s)

western blot: suitable

NCBI accession no.

NP_077330

UniProt accession no.

P09483

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CHRNA4(1137)

General description

Nicotinic acetylcholine receptors, or nAChRs, are cholinergic receptors that form ligand-gated ion channels in the plasma membranes of certain neurons. nAChR is triggered by the binding of the neurotransmitter acetylcholine (ACh) and is also opened by nicotine. After binding ACh, the nAChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. nAChRs are present in many tissues in the body: the neuronal receptors are present in the central nervous system and the peripheral nervous system; and the neuromuscular receptors are found in the neuromuscular junctions of somatic muscles. Neuronal AChR is composed of two different types of subunits: alpha (α) and beta (β).

Specificity

This antibody recognizes Nicotinic Acetylcholine Receptor α4.

Immunogen

Epitope: Extracellular Domain
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from residues of Nicotinic Acetylcholine Receptor α4.

Application

Detect Nicotinic Acetylcholine Receptor α4 using this Anti-Nicotinic Acetylcholine Receptor α4 Antibody validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Quality

Evaluated by Western Blot in rat e16 brain lysate.
Western Blot Analysis: : 1:1,000 dilution of this lot detected Nicotinic Acetylcholine Receptor α4 in 10 µg of e16 rat brain lysate.

Target description

Approx. 55 kDa

Physical form

Antigen Affinity Purified
Rabbit purified polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C for up to 12 months from date of receipt.

Analysis Note

Control
Rat e16 brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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