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528215

Sigma-Aldrich

Anti-PAI-1 Mouse mAb (MA-33B8)

liquid, ≥90% (SDS-PAGE), clone MA-33B8

Synonym(s):

Anti-Plasminogen Activator Inihibitor-1

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

MA-33B8, monoclonal

assay

≥90% (SDS-PAGE)

form

liquid

does not contain

preservative

species reactivity

rabbit, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

unmodified

Gene Information

human ... SERPINE1(5054)

General description

Purified mouse monoclonal antibody. Recognizes the ~50 kDa PAI-1 protein.
Recognizes the ~50 kDa PAI-1 protein.
This Anti-PAI-1 Mouse mAb (MA-33B8) is validated for use in Immunoblotting, Neutralization Studies for the detection of PAI-1.

Immunogen

Human
human tPA-PAI-1 complex

Application

Immunoblotting (10 µg/ml)

Neutralization Studies (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM NaCl, 50 mM sodium phosphate buffer, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Montes, R., et al. 2000. Thromb. Haemost.84, 65.
Verhamme, I., et al. 1999. J. Biol. Chem.274, 17511.
Debrock, S., and Declerck, P.J. 1997. Biochim. Biophys. Acta1337, 257.
Rapidly inactivates PAI-1 (Plasminogen Activator Inhibitor-1) to the latent form. Like the native latent form, PAI-1 inactivated by antibody was protected from cleavage by elastase. Also shown to reduce renal fibrin deposits in a rabbit model of endotoxemia. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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