Magna RIP Quad RNA-Binding Protein Immunoprecipitation Kit

RNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

RNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 48 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads.

RIP Kit capacity: 48 RNA-binding protein immunoprecipitation assays
Eight boxes containing all necessary reagents to perform 48 individual RNA-binding protein immunoprecipitation (RIP) reactions.

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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