126741
Alcalase® Enzyme, Bacillus licheniformis
Synonym(s):
Protease from Bacillus licheniformis, subtilisin A
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About This Item
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biological source
Bacillus licheniformis
Quality Level
form
liquid
specific activity
≥0.75 Anson units/mL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
activity assay: suitable
color
dark brown
application(s)
life science and biopharma
shipped in
ambient
storage temp.
2-8°C
General description
Alcalase® is a serine endopeptidase that consists primarily of subtilisin A.
Application
Alcalase® Enzyme, Bacillus licheniformis has been used in the proteolytic digestion and extraction of polysaccharides in various sponge species. It has also been used as an endoprotease enzyme in enzymatic hydrolysis for the production of fish protein hydrolysate.
Biochem/physiol Actions
Alcalase® is suitable for the hydrolysis of proteins with an optimal pH of 7.0-8.5. It is used as an additive in detergent, dehairing, and bating leather. Alcalase is also used in meat tenderizing, cheese flavor development, baked manufacture, and improving the digestibility of animal feeds. Commercial Alcalase® comprises a liquid enzymatic preparation containing 50% glycerol, 41% water, and 9% protease extract from Bacillus licheniformis.
Warning
Toxicity: Harmful (C)
Unit Definition
One unit is defined as the amount of enzyme that will release 1.0 µmol L-tyrosine from hemoglobin per min at 25°C, pH 7.5.
Reconstitution
Please refer to the vial label for lot-specific density.
Legal Information
Alcalase is a registered trademark of Novozymes Corp.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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