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07-748-AF488

Sigma-Aldrich

Anti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor™ 488 conjugate

from rabbit, ALEXA FLUOR™ 488

Synonym(s):

H4K20me1, Histone H4, Lys20 monomethylated, H4 histone family member, Lys20 monomethylated

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

rabbit

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable

NCBI accession no.

NM_001034077.4

UniProt accession no.

P62805

target post-translational modification

monomethylation (Lys20 )

Gene Information

human ... HIST2H4B(554313)

General description

Histone H4 (UniProt P62805; also known as H4 histone family member A, Histone 1 H4a) is encoded by the HIST1H4A (also known as H4/A, H4FA) gene (Gene ID 8359) in human. Histone H4 is one of the main histone proteins that contribut to the chromatin structure in both prokaryotic and eukaryotic cells. Consecutive methylation of the histone H4 Lys20 (H4K20) is dependent on nucleosome age, and different H4K20 methylation levels are found at distinct nuclear loci. H4K20 monomethylation (H4K20me1) is a repressive mark of transcribed genes, whereas H4K20 trimethylation (H4K20me3) is found in repetitive elements in the human genome. More than 80% of H4K20 is dimethylated in human cells. At least three different methyltransferases (KMTs) have been identified that can catalyze H4K20 methylation, while only lysine demethylases (KDMs) for H4K20me1, but not for H4K20me2 or H4K20me3, have been discovered. The monomethyltransferase gene Setd8 knockout in mouse and in Drosophila is lethal due to defects in cell cycle progression.

Specificity

Broad species cross-reactivity expected due to sequence homology.

Application

Anti-monomethyl Histone H4 (Lys20) Antibody, Alexa Fluor™ 488 conjugate is an antibody against monomethyl Histone H4 (Lys20) for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected histone H4 Lys20 monomethylation in HeLa cells.
The unconjugated antibody (Cat. No. 07-748) is shown to be suitable also for Immunocytochemistry, Multiplex assay, and Western blotting applications.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H4 Lys20 monomethylation in A431 cells.

Target description

~10 kDa observed.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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