07-1720
Anti-FOXO4 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Fork head domain transcription factor AFX1, forkhead box O4, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog)
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
species reactivity (predicted by homology)
rat
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FOXO4(4303)
mouse ... Foxo4(54601)
General description
Forkhead box O4 (FOXO4 or AFX) is a member of the forkhead family of transcription factors, (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. FOXO4 has similar properties to FOXO1, FOXO3, and FOXO and is associated with acute leukemia. Upon phosphorylation, FOXO4 is translocated from the cytosol to the nucleus. FOXO4 has three phosphorylation sites at Thr32, Ser197, and Ser262. There are 2 pathways, Akt and reversible acetylation, involved in negative control of FOXO4, resulting in cell survival. FOXO4 represses smooth muscle cell (SMC) differentiation by interacting and suppressing myocardin. FOXO4 also down regulates HIF-1 alpha protein levels which prevent hypoxia response.
Specificity
This antibody recognizes FOXO4 at the N-terminus.
Immunogen
Epitope: N-terminus
Synthetic peptide corresponding to the N-terminal region of FOXO4.
Application
Anti-FOXO4 Antibody is an antibody against FOXO4 for use in WB & IC.
Immunocytochemistry:
A 1:500 dilution of this antibody was used to detect FOXO4 in NIH/3T3 and A431 cells. Positive nuclear staining.
A 1:500 dilution of this antibody was used to detect FOXO4 in NIH/3T3 and A431 cells. Positive nuclear staining.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Western Blot Analysis:
1:500 dilution of this antibody was used to detect FOXO4 in mouse kidney lysate.
1:500 dilution of this antibody was used to detect FOXO4 in mouse kidney lysate.
Target description
~65 kDa
Linkage
Replaces: MABE539; MABD181
Physical form
Affinity purified
Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.
Analysis Note
Control
Mouse kidney lysate.
Mouse kidney lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Sarah M Senf et al.
American journal of physiology. Cell physiology, 300(6), C1490-C1501 (2011-03-11)
The Forkhead Box O (FOXO) transcription factors regulate diverse cellular processes, and in skeletal muscle are both necessary and sufficient for muscle atrophy. Although the regulation of FOXO by Akt is well evidenced in skeletal muscle, the current study demonstrates
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