04-035
Anti-PTEN Antibody, clone 6H2.1
clone 6H2.1, Upstate®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6H2.1, monoclonal
species reactivity
rat, human, mouse
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PTEN(5728) , TEP1(7011)
General description
The protein and lipid phosphatase PTEN (phosphatase and tensin homolog deleted on chromosome ten) has been suggested to play a role in tumorgenesis. PTEN is a tumor suppressor whose loss permits constitutive signaling through the PI3K pathway and is sufficient to cause cancer. In normal cells, in conjunction with FOXO1A and PTEN function together to restrain cell growth. PTEN metabolizes the product of PI3K phosphorylation of PI(4,5)P2 following receptor activation, creating PI(3,4,5)P3. In cells lacking PTEN, there are elevated levels of PI(3,4,5)P3, which acts as a potent second messenger promoting oncogenesis. Restoration of PTEN function is considered a novel approach to cancer therapy. Overexpression of PTEN inhibits cell migration, and expression of antisense PTEN mRNA enhances migration.
Specificity
recognizes PTEN
Immunogen
C-terminal 100 amino acids of PTEN
Epitope: C-terminal region
Application
Anti-PTEN Antibody, clone 6H2.1 detects level of PTEN & has been published & validated for use in WB, IP, IH(P).
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Quality
routinely evaluated on HeLa cell lysate
Target description
55 kDa
Linkage
Replaces: 07-016
Physical form
Format: Purified
Protein G Purified
Purified mouse IgG in storage buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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BRCAness profile of sporadic ovarian cancer predicts disease recurrence.
Wysham, WZ; Mhawech-Fauceglia, P; Li, H; Hays, L; Syriac, S; Skrepnik, T; Wright et al.
Testing null
Caroline E Nunes-Xavier et al.
The Prostate, 81(12), 838-848 (2021-06-15)
Novel immune checkpoint-based immunotherapies may benefit specific groups of prostate cancer patients who are resistant to other treatments. We analyzed by immunohistochemistry the expression of B7-H3, PD-L1/B7-H1, and androgen receptor (AR) in tissue samples from 120 prostate adenocarcinoma patients treated
Peptide inhibitors disrupt the serotonin 5-HT2C receptor interaction with phosphatase and tensin homolog to allosterically modulate cellular signaling and behavior.
Anastasio, NC; Gilbertson, SR; Bubar, MJ; Agarkov, A; Stutz, SJ; Jeng, Y; Bremer et al.
The Journal of Neuroscience null
Whole chromosome instability caused by Bub1 insufficiency drives tumorigenesis through tumor suppressor gene loss of heterozygosity.
Baker, DJ; Jin, F; Jeganathan, KB; van Deursen, JM
Cancer Cell null
Janire Mingo et al.
European journal of human genetics : EJHG, 26(8), 1180-1187 (2018-05-01)
The PTEN gene encodes a master regulator protein that exerts essential functions both in the cytoplasm and in the nucleus. PTEN is mutated in the germline of both patients with heterogeneous tumor syndromic diseases, categorized as PTEN hamartoma tumor syndrome
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