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810250P

Avanti

25-NBD Cholesterol

Avanti Polar Lipids

Synonym(s):

25-[N-[(7-nitro-2-1,3-benzoxadiazol-4-yl)methyl]amino]-27-norcholesterol

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About This Item

Empirical Formula (Hill Notation):
C33H48N4O4
CAS Number:
105539-27-3
Molecular Weight:
564.76
UNSPSC Code:
12352211
NACRES:
NA.25

assay

>99% (TLC)

form

powder

packaging

pkg of 1 × 1 mg (810250P-1mg)
pkg of 1 × 10 mg (810250P-10mg)
pkg of 1 × 5 mg (810250P-5mg)

manufacturer/tradename

Avanti Polar Lipids

shipped in

dry ice

storage temp.

−20°C

InChI

1S/C33H48N4O4/c1-20(7-6-8-21(2)36(5)28-13-14-29(37(39)40)31-30(28)34-41-35-31)25-11-12-26-24-10-9-22-19-23(38)15-17-32(22,3)27(24)16-18-33(25,26)4/h9,13-14,20-21,23-27,38H,6-8,10-12,15-19H2,1-5H3/t20-,21?,23+,24+,25+,26+,27+,32+,33-/m1/s1

InChI key

RMGLSQAGXRZCPZ-OVHOIDCTSA-N

Application

25-NBD Cholesterol is suitable:
  • as a cholesterol source in De Man, Rogosa and Sharpe (MRS) broth used for qualitative cholesterol assimilation study
  • for use in the outer lipid coating step during the preparation of fluorescently labelled lipid-coated calcium phosphate (LCP)- gemcitabine monophosphate (GMP)
  • for liposome preparation

Biochem/physiol Actions

Nitrobenzoxadiazolyl (NBD) cholesterol is a fluorescently tagged cholesterol, useful to differentiate exogenous and endogenous cholesterol in bacterial cell membrane. The NBD cholesterol is indicated as green colour in fluorescent signals.

Packaging

5 mL Amber Glass Screw Cap Vial (810250P-10mg)
5 mL Amber Glass Screw Cap Vial (810250P-1mg)
5 mL Amber Glass Screw Cap Vial (810250P-5mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Storage Class

11 - Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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In vitro assessment of pediococci-and lactobacilli-induced cholesterol-lowering effect using digitally enhanced high-performance thin-layer chromatography and confocal microscopy
Syakila RN, et al.
Analytical and Bioanalytical Chemistry, 411(6), 1181-1192 (2019)
Constitutive boost of a K+ channel via inherent bilayer tension and a unique tension-dependent modality
Iwamoto M and Oiki S
Proceedings of the National Academy of Sciences of the USA, 115(51), 13117-13122 (2018)
Gemcitabine nanoparticles promote antitumor immunity against melanoma
Zhang Y, et al.
Biomaterials, 189, 48-59 (2019)
Johan Tornmalm et al.
Scientific reports, 9(1), 18133-18133 (2019-12-04)
Protein-lipid interactions in cellular membranes modulate central cellular functions, are often transient in character, but occur too intermittently to be readily observable. We introduce transient state imaging (TRAST), combining sensitive fluorescence detection of fluorophore markers with monitoring of their dark

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