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Ascentis® Express 90 Å Biphenyl (2 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm UUHPLC Column

Synonym(s):

Ascentis® Express 90 Å Biphenyl, 2.0 μm HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Ascentis® Express 90 Å Biphenyl, 2.0 μm HPLC Column, column L × I.D. 10 cm × 2.1 mm

material

stainless steel column

Agency

suitable for USP L11

product line

Ascentis®

feature

endcapped

packaging

1 ea of

extent of labeling

7.0% Carbon loading

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

column L × I.D.

10 cm × 2.1 mm

surface area

120 m2/g

matrix

Fused-Core® particle platform
superficially porous particle

matrix active group

Biphenyl (dimethylbiphenyl) bonding phase

particle size

2.0 μm

pore size

90 Å pore size

operating pH range

2-9

separation technique

reversed phase

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General description

Ascentis® Express 90 Å Biphenyl is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 μm thick porous shell and the small overall particle size of 2 μm. The bonded, densely endcapped, dimethyl-biphenyl stationary phase provides a stable, reversed phase packing with enhanced pi-pi and mild steric interactions due to the two sequential phenyl groups bonded to the base silica. The 90 Å Biphenyl can be used for basic, acidic, or neutral compounds with alternate selectivity from C18 and is especially useful for separating peaks that elute early on C18 and other phenyl phases.

Application

UHPLC/MS Analysis of Pain Management Opiates
UHPLC/MS Analysis of SAMHSA 5 Panel on Ascentis® Express Biphenyl, 2 μm

Analysis Note

Target analytes include electron-poor molecules, aromatic or unsaturated compounds (ketones, nitriles, alkenes).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Certificates of Analysis (COA)

Lot/Batch Number
USBTE001340
USBTE001338
USBTE001326
USBTE001325
USBTE001324

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Articles

Ascentis® Express Biphenyl UHPLC and HPLC Columns

The bonded, densely endcapped, dimethyl-biphenyl stationary phase of Ascentis® Express 90 Å Biphenyl, based on fused-core particles, provides a stable, reversed phase packing with enhanced π-π and mild steric interactions due to the two sequential phenyl groups bonded to the base silica.

Related Content

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

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