56103AST
apHera™ C18 HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mm
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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material
stainless steel column
Quality Level
Agency
suitable for USP L67
product line
apHera™
packaging
pkg of 1 ea
manufacturer/tradename
apHera™
parameter
147 bar pressure (2133 psi)
4-60 °C temperature
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
matrix
polymeric
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
300 Å
operating pH
1-14
application(s)
food and beverages
separation technique
reversed phase
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General description
apHera™ reversed columns were devoloped specifically to provide the superior advantages of both silica and polystyrene columns, without the disadvantages of either. This was accomplished using a vinyl alcohol copolymer base that keeps the surface wetted even with high carbon loads. The porous structure has an average pore diameter large enough to produce ideal results for small analytes, peptides and small proteins. These columns equal silica based columns in separation efficiency with organic solvents but provide efficiency with buffered alkaline solutions not possible on silica. One of the most significant features is logicial elution order of alkylated bases where retention increases proportionately with increasing chain length.
Features of apHera™ Reversed Phase Columns
Features of apHera™ Reversed Phase Columns
- Stable vinyl copolymer base
- Stable pH range 2-12
- 300 angstrom pore size
- Stable in all organic solvents
- Expanded applications: peptides, proteins
- Efficient separation of any basic substance
- Amenability to washing and alkaline solutions
Legal Information
apHera is a trademark of Sigma-Aldrich Co. LLC
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