Skip to Content
Merck
All Photos(1)

Documents

Y2146

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, and tryptophan

Synonym(s):

Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements, Yeast media, amino acid dropout supplements

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352200
NACRES:
NA.85

Quality Level

form

powder

technique(s)

transformation: suitable

application(s)

microbiology

storage temp.

room temp

Looking for similar products? Visit Product Comparison Guide

Application

Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Preparation Note

Dissolve 1.46 g of supplement per liter of medium.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 6

1 of 6

SD Agar suitable for microbiology, NutriSelect® Basic

Millipore

84605

SD Agar

Yeast Nitrogen Base NutriSelect® Plus, suitable for microbiology

Millipore

51483

Yeast Nitrogen Base

YPD Agar

Sigma-Aldrich

Y1500

YPD Agar

3-Amino-1,2,4-triazole ≥95% (TLC)

Sigma-Aldrich

A8056

3-Amino-1,2,4-triazole

L-Tryptophan reagent grade, ≥98% (HPLC)

Sigma-Aldrich

T0254

L-Tryptophan

Sudeshna Nag et al.
Journal of cell science, 131(18) (2018-08-30)
Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing
Riddhi Atul Jani et al.
Journal of cell science, 128(17), 3263-3276 (2015-07-26)
Melanosomes are a class of lysosome-related organelles produced by melanocytes. Biogenesis of melanosomes requires the transport of melanin-synthesizing enzymes from tubular recycling endosomes to maturing melanosomes. The SNARE proteins involved in these transport or fusion steps have been poorly studied.

Articles

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

Protocols

Yeast Drop Out Bulletin. The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Although yeast can grow on a synthetic medium without any amino acids, better yield and growth rate can be achieved on richer media.

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service