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T6666

Sigma-Aldrich

Trizma® hydrochloride

pH 3.5-5.0 (0.5 M in H2O), BioXtra, ≥99.0% (titration)

Synonym(s):

TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

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About This Item

Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
1185-53-1
Molecular Weight:
157.60
Beilstein:
3675235
EC Number:
214-684-5
MDL number:
MFCD00012590
UNSPSC Code:
12352104
PubChem Substance ID:
24900398

product line

BioXtra

Quality Level

400

Assay

≥99.0% (titration)

form

powder

impurities

Insoluble matter, passes filter test

ign. residue (900 °C)

≤0.2% (as SO4)

loss

≤0.2% loss on drying, 110°C

pH

3.5-5.0 (0.5 M in H2O)

useful pH range

7.0-9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubility

H2O: 0.5 M, clear, colorless

anion traces

sulfate (SO42-): ≤0.005%

cation traces

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorption

≤0.010 at 280 in H2O at 0.5 M
≤0.015 at 260 in H2O at 0.5 M

application(s)

diagnostic assay manufacturing

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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