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T3809

Sigma-Aldrich

TRI Reagent®

BD, For processing whole blood, plasma, or serum.

Synonym(s):

DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, TRI Reagent® blood plasma serum, blood RNA extraction, blood RNA isolation, blood RNA purification, single step RNA extraction reagent, single step RNA isolation reagent, single step RNA purification reagent, total RNA extraction reagent, total RNA extraction solution, total RNA isolation reagent, total RNA isolation solution, total RNA purification reagent, total RNA purification solution, TRI Reagent® RNA Isolation Reagent

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About This Item

MDL number:
MFCD00213058
UNSPSC Code:
12352200
NACRES:
NA.52

Quality Level

200

usage

0.75 mL sufficient for 0.25 mL blood derivatives

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General description

TRI Reagent BD is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein from serum, plasma or whole blood. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA and protein. This procedure is an adaptation of the single-step method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of blood derivatives.

TRI REAGENT BD performs well with large or small sample volumes, and many samples can be simultaneously extracted. TRI Reagent BD is a mixture of guanidine thiocyanate and phenol in a mono-phase solution. When a sample of blood derivatives is lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI REAGENT BD processes 0.25 ml of blood derivatives.

This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh cells. The procedure is very effective for isolating RNA molecules of all types from 0.1 to 15 kb in length. The resulting RNA is intact with little or no contaminating DNA or protein.
TRI Reagent® is a monophasic solution of phenol and guanidinium isothiocyanate.

Application

TRI Reagent® has been used for the isolation of RNA.
The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assays, cloning and PCR.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

Features and Benefits

  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Supplementary Hazards

EUH032

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

174.2 °F - closed cup

Flash Point(C)

79 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

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