SRP3069
IGF-I human
Animal-component free, recombinant, expressed in E. coli, suitable for cell culture
Synonym(s):
IGF-IA, Somatamedin C
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥95% (HPLC)
form
lyophilized powder
potency
0.1-10 ng/mL ED50/EC50
mol wt
~7.6 kDa
packaging
pkg of 100 μg
pkg of 50 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg endotoxin, tested
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IGF1(3479)
Related Categories
General description
IGFs (insulin like growth factors) are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-I expression is regulated by growth hormone. IGF-I signal through the tyrosine kinase type I receptor (IGF-IR). Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and three intra-molecular disulfide bonds.
Application
IGF-I human has been used:
- as positive control in the quantification of growth factors in adipose-derived stem cells using enzyme-linked immunosorbent assay (ELISA)
- as a medium supplement for trunk crestosphere cultures
- in stimulation protein kinase B (Akt) methylation in human embryonic kidney cells (HEK293) and human melanoma cell line(A375)
In broiler chicks, IGF-I human has been used to study the effect of fadrozole and IGF-1 on female-to-male sex reversal and body weight.
Biochem/physiol Actions
The IGFs (insulin like growth factors) are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF1 is an essential component for embryonic and postnatal skeletal development. Absence of IGF1 in mice negatively affects bone development, causes hypomineralization of skeletons and growth plate disturbances. It is also associated with cancer progression. It can suppress cell death and provides resistance to chemotherapy as well as radiation therapy.
Sequence
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
Physical form
Lyophilized with no additives.
Reconstitution
It is recommended to centrifuge the vial prior to reconstitution. A stock solution may be prepared by reconstituting in sterile 0.2% acetic acid (recommended) to a concentration of 1.0 mg/ml. The stock solution may be diluted further using aqueous buffers.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Resveratrol suppresses IGF-1 induced human colon cancer cell proliferation and elevates apoptosis via suppression of IGF-1R/Wnt and activation of p53 signaling pathways.
Vanamala J
BMC Cancer, 10, doi: 10-doi: 10 (2010)
AKT methylation by SETDB1 promotes AKT kinase activity and oncogenic functions
Guo J, et al.
Nature Cell Biology, 21(2), 226-226 (2019)
J L Anderson et al.
Journal of dairy science, 98(8), 5709-5719 (2015-06-16)
The objective of this study was to determine if increased dietary fat from dried distillers grains with solubles (DDGS) in diets of growing heifers affected metabolic profile, plasma fatty acid profile, and reproductive maturation. Thirty-three Holstein heifers (133±18 d of
Organization and sequence of the human insulin-like growth factor I gene. Alternative RNA processing produces two insulin-like growth factor I precursor peptides.
Rotwein P
The Journal of Biological Chemistry, 261, 4828-4832 (1986)
Sustained IGF-1 secretion by adipose-derived stem cells improves infarcted heart function
Bagno LL, et al.
Cell Transplantation, 25(9), 1609-1622 (2016)
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