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SAB4200693

Sigma-Aldrich

Anti-CNPase antibody, Mouse monoclonal

clone 11-5B, purified from hybridoma cell culture

Synonym(s):

2′,3′-cyclic-nucleotide 3′-phosphodiesterase, CNP, CNPase

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

200

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

11-5B, monoclonal

form

buffered aqueous solution

mol wt

antigen 46 kDa (CNP1)
 48 kDa (CNP2)

species reactivity

pig (pig origin but not guinea pig), sheep, rat, bovine, rabbit, canine, human, mouse

concentration

~1 mg/mL

technique(s)

immunoblotting: 5-10 μg/mL using whole rat brain extract

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CNP(1267)

General description

Anti-CNPase antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the 11-5B hybridoma produced by the fusion of mouse myeloma cells and splenocytes from RBF/Dn mouse immunized with human 2α,3α-cyclic nucleotide 3α-phosphodiesterase. CNPase (2α,3α-cyclic nucleotide 3α-phosphodiesterase), also known as CNP, is a membrane-anchored enzyme within the myelin sheath. CNPase is primarily a heterodimer of approximately 94 kDa. The heterodimer consists of a varied proportion of CNPase isoforms CNP1 (46 kDa) and CNP2 (48 kDa).

Immunogen

Human 2′,3′-cyclic nucleotide 3′-phosphodiesterase

Application

Anti-CNPase antibody, Mouse monoclonal has been used in immunoblotting, immunohistochemistry.

Biochem/physiol Actions

CNPase (2α,3α-cyclic nucleotide 3α-phosphodiesterase), interacts with cytoskeletal proteins, cholesterol-rich cell membranes, nucleotides and various other proteins. Abnormalities in CNPase expression cause perturbations in myelin structure and are associated with neurological diseases such as schizophrenia and multiple sclerosis. It was shown that CNPase can bind Gag structural protein and prevent the assembly of HIV-1 particle, demonstrating the anti-viral activity of CNPase.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gravel M., et al.,
Journal of Neuroscience Research, 87, 1069-179 (2009)
Thompson RJ.,
Biochemical Society Transactions, 20, 621-626 (1992)
2', 3'-cyclic nucleotide 3'-phosphodiesterase, an oligodendrocyte-Schwann cell and myelin-associated enzyme of the nervous system.
Sprinkle T J.
Critical Reviews in Neurobiology, 4(3), 235-301 (1989)
Inhibition of HIV-1 particle assembly by 2?, 3?-cyclic-nucleotide 3?-phosphodiesterase
Wilson S J, et al.
Cell host & microbe, 12(4), 585-597 (2012)
Lee J., et al.,
The Journal of Cell Biology, 170, 661-73 (2005)
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