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P9249

Sigma-Aldrich

Monoclonal Anti-p53−Biotin antibody produced in mouse

~1 mg/mL, clone DO-1, ascites fluid, buffered aqueous solution

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About This Item

MDL number:
MFCD01864735
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

biotin conjugate

antibody form

ascites fluid

antibody product type

primary antibodies

clone

DO-1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~53 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

flow cytometry: suitable
immunocytochemistry: 5-10 μg/mL using human A431
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1 μg/mL using human A431 cell extracts

isotype

IgG2a

UniProt accession no.

P04637

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)

Related Categories

General description

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Immunogen

recombinant human wild type p53. The epitope resides beween amino acids 20-25 of human p53.

Application

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

Biochem/physiol Actions

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree”freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)
Chromosomal Instability andTumors Promoted by DNAHypomethylation
Amir Eden
Science, 30 (2003)
Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)
Cooper GM.
The Cell: A Molecular Approach (2000)
Suppression of p53 potentiates chemosensitivity in nutrient-deprived cholangiocarcinoma cells via inhibition of autophagy
Fei Hu, et al.
Oncology Letters, 14(2) (2017)
View All Related Papers

Articles

p53: DNA Damage Response and Cancer

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

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